SUPEROXIDE-PRODUCING LIPOPROTEIN AS A FACTOR OF TUMOR-GROWTH SUPPRESSION, ENHANCEMENT OF LEUKOCYTE NUMBER, AND ACCELERATION OF CELL-DIVISION IN CULTURE
Ma. Simonyan et al., SUPEROXIDE-PRODUCING LIPOPROTEIN AS A FACTOR OF TUMOR-GROWTH SUPPRESSION, ENHANCEMENT OF LEUKOCYTE NUMBER, AND ACCELERATION OF CELL-DIVISION IN CULTURE, Biochemistry, 61(9), 1996, pp. 1117-1120
Intact rats were injected intraperitoneally four times with a superoxi
de-producing lipoprotein which was isolated from human blood serum, pu
rified, and activated by Fe3+ (suprol, 150 mu g/ml), and after 24 h an
increase in peripheral blood leukocyte number by only 8-10% was obser
ved. An injection of the active suprol into animals three days after i
noculation of the Pliss lymphosarcoma or the M-1 sarcoma caused an inc
rease in the leukocyte number approximating that of the intact (health
y) animals. However, the growth of the M-1 sarcoma and the Pliss lymph
osarcoma decreased by 31 +/- 4 and 52 +/- 2%, respectively. An additio
n of active suprol (15 mu g/ml) into chick embryo cell culture during
the steady-state growth phase stimulated the cell growth by 52.1%. Con
centrations of active suprol above 50 mu g/ml caused the complete lysi
s of the cells in culture. In control experiments Cu,Zn-superoxide dis
mutase suppressed the suprol-stimulated cell growth in the actively pr
oliferating culture, while inactive (native) suprol virtually failed t
o enhance leukocyte number, to suppress growth of the above-mentioned
tumors, and to stimulate cell growth in culture. These results suggest
that superoxide radicals are likely to participate in these processes
.