A new procedure for purification of endoglucanase from the Penicillium
verruculosum cellulase complex is described. The method involves ammo
nium sulfate precipitation and immunosorbent chromatography. Character
istics of the enzyme purified by the new method were compared with tho
se of the enzyme prepared by the conventional procedure. The method si
gnificantly reduces the cost and time required for isolation and purif
ication of cellulases.