Hp. Yang et al., LATE EMBRYOGENESIS ABUNDANT PROTEIN IN ARABIDOPSIS-THALIANA HOMOLOGOUS TO CARROT ECP31, Physiologia Plantarum, 98(3), 1996, pp. 661-666
Using carrot ECP31 cDNA as a probe, we isolated AtECP31 cDNA from a se
ed library of Arabidopsis thaliana. The AtECP31 cDNA contained an open
reading frame of 256 amino acids. Its deduced amino acid sequence sho
wed more than 60% identity with those of DCECP31, ZMRAB28 and GHLEA D-
34 which are late embryogenesis abundant (LEA) proteins of carrot, mai
ze and cotton, respectively. Northern blot analysis showed that the tr
anscripts of AtECP31 accumulated specifically in mature seeds. The res
ults suggest that AtECP31 cDNA, which encodes a LEA protein in group 5
, might be involved in maturation and desiccation tolerance of seeds.
Restriction fragment length polymorphism (RFLP) mapping showed that At
ECP31 gene was present on chromosome 3. Cleaved amplified polymorphic
sequence (CAPS) mapping allowed us to map more accurately the AtECP31
gene in the region of 28.3-29.6 cM of chromosome 3.