LAMININ INHIBITS AMYLOID-BETA-PEPTIDE FIBRILLATION

Laminin, an important extracellular matrix component is induced by bra in injury and colocalizes with amyloid-beta-peptide (A beta) deposits in Alzheimer brains. We report here that laminin inhibits amyloid fibr il formation as determined by thioflavin T fluorescence spectroscopy a nd electron microscopic examination. The inhibition of amyloid formati on by laminin was concentration dependent and was observed at a lamini n concentration of 300 nM, corresponding to a laminin/A beta protein m olar ratio of 1:800. The potential effect of laminin, may prove import ant to inhibit AP fibrillogenesis in vivo, specifically at the level o f cerebral blood vessels.