M. Petrovic et al., THE DNA-BINDING AFFINITY OF RAT-LIVER NUCLEOPROTEINS TO THE ALPHA(1)-ACID GLYCOPROTEIN GENE, Biochemistry and molecular biology international, 40(4), 1996, pp. 741-749
Transcriptional regulation and binding interactions between soluble nu
cleoproteins and the distal regulatory element (DRE) of the rat alpha
1-acid glycoprotein (alpha 1-AGP) gene were examined in the liver of r
ats during the acute-phase response. Our results show that the elevati
on of the alpha 1-AGP gene transcription activity in acute phase liver
relies basically on an increase in the binding-affinity of the consti
tutive soluble nucleoproteins with molecular masses 35 and 45 kD, enha
ncing their capabillity to bind to the distal regulatory element (DRE)
of alpha 1-AGP gene. On the basis of in vitro phosphorylation/dephosp
holylation experiments we discuss that the role of these proteins duri
ng alpha(1)-AGP transcription may be dependent on their behavior as ph
osphoproteins. The 35kD nucleoprotein that displayed an acute-phase in
ducible affinity to bind DRE was indentified as a C/EBP beta isoform.