R. Petter et Kj. Kwonchung, DISRUPTION OF THE SNF1 GENE ABOLISHES TREHALOSE UTILIZATION IN THE PATHOGENIC YEAST CANDIDA-GLABRATA, Infection and immunity, 64(12), 1996, pp. 5269-5273
The SNF1 gene product, a serine/threonine protein kinase, is a global
regulatory protein which has been isolated from several organisms, In
Saccharomgces cerevisiae the SNF1 gene product is essential for the de
repression of glucose repression since snf1 strains are unable to util
ize sucrose, galactose, maltose, melibiose, or nonfermentable carbohyd
rates. Moreover, the SNF1 gene product was suggested to interact with
additional regulatory pathways and to affect the expression of multipl
e target genes as reflected by the pleiotropic nature of the snf1 muta
tion. Here,ve report the characterization of the SNF1 homolog of Candi
da glabrata, a pathogenic yeast phylogenetically related to S. cerevis
iae, The carbon utilization spectrum of C. glabrata is considerably na
rrower than that of other pathogenic yeasts, and the majority of the s
trains utilize solely glucose and trehalose from among 20 of the most
commonly tested carbohydrates. Disruption of the C. glabrata SNF1 homo
log resulted in the loss of the ability to utilize trehalose, indicati
ng that even in an organism With such a limited carbon utilization spe
ctrum, the regulatory mechanism governing catabolic repression is pres
erved.