DETERMINATION OF KINETIC-PARAMETERS OF EPITOPE-PARATOPE INTERACTION BASED ON SOLID-PHASE BINDING - AN INEXPENSIVE ALTERNATE TO BIOSPECIFIC INTERACTION ANALYSIS

A method has been developed for biospecific interaction analysis betwe en antigen and antibody using solid phase binding approach. Real time kinetics between monoclonal antibody and human chorionic gonadotropin have been studied. Kinetic constants of the bimolecular reaction are d etermined Affinity constants measured by several independent methods h ave been found to be relatively consistent Convenient and simple proce dures to determine affinity constant, K-on and K-off of monoclonal ant ibody-human chorionic gonadotropin interaction using binding of [I-125 ]hCG to immobilized monoclonal antibody are presented. Values obtained compare well with these obtained using surface plasmon resonance tech nology, making this method a viable alternative.