CUCUMBER MOSAIC-VIRUS FROM IXORA - INFECTIOUS RNA TRANSCRIPTS CONFIRMDEFICIENCY IN SATELLITE SUPPORT AND UNUSUAL SYMPTOMATOLOGY

A cucumber mosaic virus isolate from an Ixora plant originating in the Philippines and first described 20 years ago (CMV-Ix) by Waterworth a nd Povish differs from other characterized CMV strains in its anomalou s satellite support and in several biological and molecular properties . We describe the preparation of infectious transcripts from cloned co mplementary DNA and the characterization of progeny virus. The results confirm that CMV-Ix has a more limited host range than most CMV isola tes. Virions of CMV-Ix are smaller than those of the control CMV-S, in dependent of the type of negative stain used. Furthermore, CMV-Ix from transcripts supports the replication of the satellite T-CARNA 5 but n ot of D-CARNA 5.