Gt. Kim et al., CHARACTERIZATION OF CATABOLITE REPRESSION AND THE PROMOTER OF THE ALGINATE LYASE GENE (ALY) FROM PSEUDOMONAS SP, Biotechnology letters, 18(11), 1996, pp. 1271-1276
Expression of the alginate lyase gene (aly) from Pseudomonas sp. W7 in
Escherichia coli was very high (about 40%) and regulated by catabolit
e repression. The aly promoter was independent upon activation by cycl
ic AMP-cAMP receptor protein (cAMP-CRP) complex. When the aly promoter
was removed by the construction of pKAL22, alginate lyase, which was
under the control of lac promoter in E. coli, was markedly decreased (
about 10%). This result confirmed that the aly promoter has stronger e
xpression system in E. coli than lac promoter.