CHARACTERIZATION OF CATABOLITE REPRESSION AND THE PROMOTER OF THE ALGINATE LYASE GENE (ALY) FROM PSEUDOMONAS SP

Authors
KIM GT KIM JM YU JH KONG IS
Citation
Gt. Kim et al., CHARACTERIZATION OF CATABOLITE REPRESSION AND THE PROMOTER OF THE ALGINATE LYASE GENE (ALY) FROM PSEUDOMONAS SP, Biotechnology letters, 18(11), 1996, pp. 1271-1276
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
11
Year of publication
1996
Pages
1271 - 1276
Database
ISI
SICI code
0141-5492(1996)18:11<1271:COCRAT>2.0.ZU;2-4
Abstract
Expression of the alginate lyase gene (aly) from Pseudomonas sp. W7 in Escherichia coli was very high (about 40%) and regulated by catabolit e repression. The aly promoter was independent upon activation by cycl ic AMP-cAMP receptor protein (cAMP-CRP) complex. When the aly promoter was removed by the construction of pKAL22, alginate lyase, which was under the control of lac promoter in E. coli, was markedly decreased ( about 10%). This result confirmed that the aly promoter has stronger e xpression system in E. coli than lac promoter.