K. Aiura et al., INTERACTION WITH AUTOLOGOUS PLATELETS MULTIPLIES INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR PRODUCTION IN MONONUCLEAR-CELLS, The Journal of infectious diseases, 175(1), 1997, pp. 123-129
The effect of activated platelets on cytokine production by human peri
pheral blood mononuclear cells (PBMC) was investigated. When PBMC were
coincubated with activated autologous platelets amid lipopolysacchari
de (LPS, 50-100 pg/mL) for 8 h, the production of interleukin (IL)-1 a
lpha increased 11- to 18-fold and tumor necrosis factor (TNF)-alpha 3-
to 5-fold compared with PBMC without platelets. Activated platelets i
n a dual-chamber well that prevented platelet-PBMC contact but permitt
ed passage of soluble factors enhanced IL-1 alpha production (P < .01)
. Platelet-PBMC contact in the chamber resulted in a further enhanceme
nt of IL-1 alpha production. These data suggest that platelet-PBMC int
eraction, both directly and with platelet-derived factors, enhances pr
oduction of shock-producing IL-1 alpha and TNF-alpha, albeit different
ly. The interaction of platelets with monocytes may play an important
role in the pathophysiology of sepsis and disseminated intravascular c
oagulation.