COMPARATIVE THERMODYNAMIC STABILITY OF BOVINE AND PIGEON HEMOGLOBINS BY INTERACTION WITH SODIUM N-DODECYL SULFATE

Authors
BORDBAR AK MOOSAVIMOVAHEDI AA SABOURY AA
Citation
Ak. Bordbar et al., COMPARATIVE THERMODYNAMIC STABILITY OF BOVINE AND PIGEON HEMOGLOBINS BY INTERACTION WITH SODIUM N-DODECYL SULFATE, Thermochimica acta, 287(2), 1996, pp. 343-349
Citations number
21
Categorie Soggetti
Chemistry Analytical
Journal title
Thermochimica acta → ACNP
ISSN journal
00406031
Volume
287
Issue
2
Year of publication
1996
Pages
343 - 349
Database
ISI
SICI code
0040-6031(1996)287:2<343:CTSOBA>2.0.ZU;2-1
Abstract
The binding of sodium n-dodecyl sulphate (SDS) to pigeon and bovine ha emoglobin has been studied at pH 6.4 and 25 degrees C by equilibrium d ialysis and titration microcalorimetry techniques. The thermodynamic p arameters of these interactions have been determined by application of the Wyman binding potential concept and interpreted from a structural viewpoint. A comparison of the results shows that bovine haemoglobin has more thermodynamic stability than pigeon haemoglobin. From this, a reason for the high oxygen affinity of avian haemoglobin is proposed.