KINETICS OF OXIDATION OF ETHANOLAMIDES OF ARACHIDONIC (ANANDAMIDE) AND EICOSAPENTAENOIC ACIDS BY SOYBEAN 15-LIPOXYGENASE

Authors
BOBROV MY ARCHAKOV AV KOGTEVA GS FOMINAAGEEVA EV ZINCHENKO GN GRETSKAYA NM KUKLEV DV BEZUGLOV VV
Citation
My. Bobrov et al., KINETICS OF OXIDATION OF ETHANOLAMIDES OF ARACHIDONIC (ANANDAMIDE) AND EICOSAPENTAENOIC ACIDS BY SOYBEAN 15-LIPOXYGENASE, Bioorganiceskaa himia, 22(10-11), 1996, pp. 875-877
Citations number
14
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
22
Issue
10-11
Year of publication
1996
Pages
875 - 877
Database
ISI
SICI code
0132-3423(1996)22:10-11<875:KOOOEO>2.0.ZU;2-Q
Abstract
Oxidation of arachidonic acid ethanolamide (anandamide) and eicosapent aenoic acid ethanolamide by soybean 15-lipoxygenase was studied by UV spectroscopy. The K-M values for both amides (3.2 and 4.9 mu M, respec tively) proved to be similar to that for arachidonic acid. Products of double lipoxygenase oxidation were identified in the reaction mixture at an enzyme-substrate ratio of 500 : 1.