MIXED BACKBONE OLIGONUCLEOTIDES CONTAINING INTERNUCLEOTIDIC PRIMARY PHOSPHORAMIDATE LINKAGES

Authors
DEVLIN T IYER RP JOHNSON S AGRAWAL S
Citation
T. Devlin et al., MIXED BACKBONE OLIGONUCLEOTIDES CONTAINING INTERNUCLEOTIDIC PRIMARY PHOSPHORAMIDATE LINKAGES, Bioorganic & medicinal chemistry letters, 6(22), 1996, pp. 2663-2668
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
6
Issue
22
Year of publication
1996
Pages
2663 - 2668
Database
ISI
SICI code
0960-894X(1996)6:22<2663:MBOCIP>2.0.ZU;2-7
Abstract
Mixed backbone oligonucleotides (MBOs) which contain segments of prima ry phosphoramidate linkages (PO-NH2) in conjunction,with either phosph oric diesters (PO), or phosphorothioates (PS) were prepared. Thermal d enaturation of the duplexes with RNA and DNA reveal that they form sta ble duplexes which display cooperative melting profiles. Preliminary s tability studies reveal that the PO-NH2 linkage is resistant to serum nucleases. Thus, these MBOs represent novel antisense molecules. Copyr ight (C) 1996 Elsevier Science Ltd