DISTRIBUTION OF THE STRESS-RELATED ANIONIC PEROXIDASES IN DIFFERENT CUCUMBER ORGANS

The distribution of the stress-related anionic peroxidase (srPRX) acti vity was investigated in various organs of cucumber (Cucumis sativus L . cv. Laura) during their development by activity staining and immunob lotting. Ln shoots, including cotyledon, leaf, stem and tendril, three stress-related peroxidase isoenzymes were present, particularly in ol d ones. The PRX1 was the only srPRX isoenzyme found in both young and old roots. As fruits became mature, srPRX activity increased dramatica lly and was particularly enriched in the external parts of the fruit. The PRX1 isoenzyme was highly accumulated in the course of seed germin ation, while the absence of other two srPRX isoenzymes (PRX2 and PRX3) was recorded. The possible function of the srPRX is discussed, with r espect to this spatio-temporal distribution.