USE OF THE LACZ REPORTER GENE AS AN INTERNAL CONTROL FOR GUS ACTIVITYIN MICROPROJECTILE BOMBARDED PLANT-TISSUE

Citation
G. Hull et al., USE OF THE LACZ REPORTER GENE AS AN INTERNAL CONTROL FOR GUS ACTIVITYIN MICROPROJECTILE BOMBARDED PLANT-TISSUE, PLANT SCI, 120(2), 1996, pp. 153-160
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
120
Issue
2
Year of publication
1996
Pages
153 - 160
Database
ISI
SICI code
0168-9452(1996)120:2<153:UOTLRG>2.0.ZU;2-#
Abstract
A protocol is described for simultaneous histochemical detection of La cZ and Gus activity in plant tissues after microprojectile bombardment . The suitability of two different Gus substrates (Salmon-glcA, Magent a-beta-D-glcA) is compared. This detection system is used to assay the number of cells expressing either or both reporter gene. This techniq ue is used as a qualitative assay to demonstrate the tissue specificit y of a Hrgp promoter in maize embryos, and to measure ABA responsivene ss of a Lea promoter in Arabidopsis. The promoter to be studied is lin ked to the gus reporter gene and the lacZ reporter gene linked to the CaMV 35S promoter is used as a constitutive internal control. The use of an internal control drastically reduces the data variation inherent to microprojectile bombardment.