P. Joling et al., IMMUNOHISTOCHEMICAL DEMONSTRATION OF CELLULAR ANTIGENS OF THE CAT DEFINED BY ANTI-HUMAN ANTIBODIES, Veterinary immunology and immunopathology, 53(1-2), 1996, pp. 115-127
A panel of monoclonal antibodies (mAb) and some polyclonal rabbit sera
directed against human antigens were studied on cryostat tissue secti
ons of three cats using immunohistochemistry. Reactivity of the antibo
dies was tested on feline tonsil, intestine, thymus, lymph node and sp
leen with a three-step immunoperoxidase technique and compared with re
actions on human thymus, lymph node and spleen. From a total of 95 ant
ibodies, 28 gave reactivity comparable with that in human tissues. The
remaining antibodies gave none or miscellaneous results. The positive
reactions in the cat included antibodies directed to adhesion molecul
es (VLA-2 and VLA-L), to natural killer (NK) cells (CD56, CD57 and NCA
M), to complement receptor CR1, to proliferation marker Ki-67 (MIB-1),
to endothelial antigens (EN-4, PAL-E and von Willebrand factor) and t
o structural proteins like vimentin, desmin, collagen type IV and cyto
keratin. The identification of these cross-reacting antibodies extends
the spectrum of immunological reagents that are now available for the
cat, and will thus contribute to the study of the feline immune syste
m.