SEQUENCE-ANALYSIS OF AN INTERNAL 9.72-KB SEGMENT FROM THE 30-KB DENITRIFICATION GENE-CLUSTER OF PSEUDOMONAS-STUTZERI

The DNA segment was sequenced that links the nir-nor and nos gene clus ters for denitrification of Pseudomonas stutzeri ATCC 14405. Of 10 pre dicted gene products, four are putative membrane proteins. Sequence si milarity was detected with the subunit III of cytochrome-e oxidase (OR F175), PQQ3 of the biosynthetic pathway for pyrrolo-quinoline quinone (ORF393), S-adenosylmethionine-dependent uroporphyrinogen-III C-methyl transferase (ORF278), the cytochrome cd(1) nitrite reductase and the N irF protein involved in the biosynthesis of heme d(1) (ORF507), LysR t ype transcriptional regulators (ORF286), short-chain alcohol dehydroge nases (ORF247), and a hypothetical protein, YBEC, of Escherichia coli (ORF57). The current data together with previous work establish a cont iguous DNA sequence of 29.2 kb comprising the supercluster of nos-nir- nor genes for denitrification in this bacterium.