Am. Ueno et al., A LOW, ADAPTIVE DOSE OF GAMMA-RAYS REDUCED THE NUMBER AND ALTERED THESPECTRUM OF S1(-) MUTANTS IN HUMAN-HAMSTER HYBRID A(L) CELLS, Mutation research, 358(2), 1996, pp. 161-169
We examined the effects of a low, adaptive dose of Cs-137-gamma-irradi
ation (0.04 Gy) on the number and kinds of mutants induced in A(L) hum
an-hamster hybrid cells by a later challenge dose of 4 Gy. The yield o
f S1(-) mutants was significantly less (by 53%) after exposure to both
the adaptive and challenge doses compared to the challenge dose alone
. The yield of hprt(-) mutants was similarly decreased. Incubation wit
h cycloheximide (CX) or 3-aminobenzamide largely negated the decrease
in mutant yield. The adaptive dose did not perturb the cell cycle, was
not cytotoxic, and did not of itself increase the mutant yield above
background. The adaptive dose did, however, alter the spectrum of S1(-
) mutants from populations exposed only to the adaptive dose, as well
as affecting the spectrum of S1(-) mutants generated by the challenge
dose. The major change in both cases was a significant increase in the
proportion of complex mutations compared to small mutations and simpl
e deletions.