IDENTIFICATION OF CYTOKERATIN SUBSPECIES ALTERED IN RAT EXPERIMENTAL ESOPHAGEAL TUMORS BY SUBTRACTIVE CLONING

Citation
Dy. Wang et al., IDENTIFICATION OF CYTOKERATIN SUBSPECIES ALTERED IN RAT EXPERIMENTAL ESOPHAGEAL TUMORS BY SUBTRACTIVE CLONING, Cancer letters, 108(1), 1996, pp. 119-127
Citations number
28
Categorie Soggetti
Oncology
Journal title
ISSN journal
03043835
Volume
108
Issue
1
Year of publication
1996
Pages
119 - 127
Database
ISI
SICI code
0304-3835(1996)108:1<119:IOCSAI>2.0.ZU;2-U
Abstract
By using the subtractive hybridization method, two complementary DNA c lones differently expressed in rat normal esophageal epithelium and sq uamous cell carcinoma induced by administration of precursors of N-nit rososarcosine ethyl eater were isolated. A rat homologue of the human 50-kDa type I cytokeratin 14 was cloned for the first time and shown t o be expressed preferentially in squamous cell papillomas and carcinom as, whereas it was weakly expressed or absent in normal squamous epith elial cells and in hyperplastic lesions. A rat homologue of the mouse 57-kDa type II cytokeratin showed strong expression in both normal and tumor tissues. These results are well consistent with the reported al teration of keratin subspecies in human esophageal cancers, therefore, encouraging us to use this experimental system as a model for human e sophageal carcinogenesis.