SERUM CREATINE-KINASE ENZYME LEVELS IN THE EARLY DIAGNOSIS OF SPERMATIC CORD TORSION

Delay in the diagnosis of spermatic cord torsion (SCT) is still a sign ificant cause of testicular loss in children. The aim of this experime ntal study was to assess the diagnostic Value of serum creatine kinase (CK) in the early period following SCT. Forty male rats were assigned randomly into five similar groups: group A, control; group B, sham, r ight testis exposed, manipulated, and blood sampling at 6th h; group C , right SCT, blood sampling at 2nd h; group D, right SCT, blood sampli ng at 4th h; and group E, right SCT, blood sampling at 6th h. CK and i ts isoenzymes were measured in the sera of all animals. All testes wer e removed and examined histopathologically. Significant increases in s erum CK levels compared to control and sham groups were observed at 4 and 6 h following SCT. The major increase in CK was observed in the CK -MM isoenzyme fraction. Histologic pictures showed varying degrees of edema, Vascular congestion, and hemorrhage in the testicular tissue, b ut no necrosis in any of the study groups. These results showed that s erum CK levels in rats in the early period following SCT increase sign ificantly before necrosis of testicular tissue. This may be of value a s a diagnostic test, to corroborate findings from clinical studies.