DETECTION OF WHEAT (TRITICUM-AESTIVUM L) TRITICUM-TIMOPHEEVII ZHUK ADDITION AND SUBSTITUTION AND WHEAT RYE TRANSLOCATION BY C-BANDING AND IN-SITU HYBRIDIZATION/
Mm. Langne et al., DETECTION OF WHEAT (TRITICUM-AESTIVUM L) TRITICUM-TIMOPHEEVII ZHUK ADDITION AND SUBSTITUTION AND WHEAT RYE TRANSLOCATION BY C-BANDING AND IN-SITU HYBRIDIZATION/, Novenytermeles, 45(3), 1996, pp. 237-245
Alien chromosomes and chromosome segments have been demonstrated in ge
netic material produced in the Department of Genetics of the Agricultu
ral Research Institute, Martonvasar and in Martonvasar wheat varieties
using two cytogenetical methods, i.e. C-banding and in situ hybridiza
tion. The advantage of C-banding is that individual chromosomes can be
clearly identified by this method, but when applying in situ hybridiz
ation shorter chromosome segments built into an alien genome can be re
cognized, which do not carry typically stained bands. In addition, the
translocation breakpoint in an alien translocation can be exactly det
ermined using in situ hybridization. A 6G Triticum aestivum L./T. timo
pheevi Zhuk. addition line and a 6G(6B) 1: timopheevii Zhuk.(T. aestiv
um L.) substitution line were identified in the progenies from T. aest
ivum L. x T. timopheevii Zhuk. crossing. The 44-chromosome 6G addition
line is a BC2F1 progeny of the T. timopheevii Zhuk. x. T. aestivum L.
cv. Mv 9 krl hybrid (Farshadfar et al. 1994) backcrossed with the whe
at variety Mv 9. The 6G(6B) disomic substitution line was found among
selected 42-chromosome progenies of a T. aestivum L. cv. Fleischmann 4
81 x T. timopheevii Zhuk. amphiploid (Belea 1961) backcrossed once wit
h the wheat variety Mironovskaya 808 and three times with the wheat va
riety Mv 14 and subsequently self-pollinated. The 1B/1R wheat/rye tran
slocation occurring in Martonvasar wheat varieties was demonstrated by
both C-banding and in situ hybridization. There was no difference in
the size of the translocated rye chromosome arms among Martonvasar whe
at varieties of different quality. The translocation breakpoint was at
the centromere in each case.