Nd. Huebert et al., SIMULTANEOUS MEASUREMENT OF DOLASETRON AND ITS MAJOR METABOLITE, MDL-74,156, IN HUMAN PLASMA AND URINE, Journal of chromatography B. Biomedical applications, 685(2), 1996, pp. 291-297
Citations number
10
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A selective and sensitive analytical method for the simultaneous measu
rement of dolasetron (I) and its major metabolite, MDL 74,156 (II), in
human plasma and urine samples has been developed using a structural
analogue, MDL 101,858, as internal standard (I.S.). The compounds were
extracted from plasma and urine using solvent extraction after the ad
dition of the I.S. Chromatographic separation was carried out on a rev
ersed-phase HPLC column and detection and quantification was by fluore
scence with excitation and emission wavelengths of 285 and 345 nm, res
pectively. Linear responses were obtained over concentration ranges of
5 to 1000 pmol/ml for plasma samples and 20 to 1000 pmol/ml for urine
samples with correlation coefficients for the calibration curves exce
eding 0.999 in all cases. Intra-day and inter-day reproducibility yiel
ded limits of quantification of 10 pmol/ml for I and 5 pmol/ml for II
in plasma and 50 pmol/ml for I and II in urine. The method has been ap
plied to the simultaneous analysis of both compounds in plasma and uri
ne samples coming from clinical pharmacokinetic studies.