A NOVEL HEME PROTEIN THAT ACTS AS A CARBON MONOXIDE-DEPENDENT TRANSCRIPTIONAL ACTIVATOR IN RHODOSPIRILLUM-RUBRUM

Authors
AONO S NAKAJIMA H SAITO K OKADA M
Citation
S. Aono et al., A NOVEL HEME PROTEIN THAT ACTS AS A CARBON MONOXIDE-DEPENDENT TRANSCRIPTIONAL ACTIVATOR IN RHODOSPIRILLUM-RUBRUM, Biochemical and biophysical research communications, 228(3), 1996, pp. 752-756
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
228
Issue
3
Year of publication
1996
Pages
752 - 756
Database
ISI
SICI code
0006-291X(1996)228:3<752:ANHPTA>2.0.ZU;2-L
Abstract
The gene coding for a carbon monoxide-dependent transcriptional activa tor (cooA) in Rhodospirillum rubrum has been expressed in E. coli, and the recombinant CooA has been purified. CooA contains b-type heme whi ch may act as a CO sensor in vivo. CO-bound CooA was formed when reduc ed CooA was reacted with CO, but not in the case of oxidized CooA. Coo A is the first example of the heme protein acting as a DNA-binding tra nscriptional activator. (C) 1996 Academic Press, Inc.