PREDICTION OF AFFINITIES OF PEPTIDES FOR CALMODULIN

A simple method has been developed to predict the calmodulin-binding d omains of peptides and the affinities of the peptides for calmodulin b ased on a model for the calmodulin-binding domains of peptides propose d in a previous paper and the literature data of calmodulin-binding pe ptides. The effects of hydrophobicities, alpha-helix-forming tendencie s, and basicities of peptides on their affinities for calmodulin were studied. Model peptides were designed to examine the calmodulin-bindin g domain model and the prediction method. The determined dissociation constants of the complexes formed from the model peptides and calmodul in are in good agreement with the predicted results.