ANTIMICROBIAL AND IMMUNOLOGICAL ACTIVITY OF ETHANOL EXTRACTS AND FRACTIONS FROM ISOPYRUM THALICTROIDES

The antimicrobial and immunological properties of ethanol extracts, no n-alkaloid, tertiary alkaloid and quaternary alkaloid fractions, obtai ned from roots and aerial parts of Isopyrum thalictroides were examine d. The non-alkaloid fraction from aerial parts inhibited the growth of seven test microorganisms and was the most effective suppresser of cl assical pathway (CP) complement activity in normal human serum (NHS) a nd guinea pig serum (GPS). The alkaloid fractions, containing quaterna ry alkaloids expressed suppressive effect on mitogen-induced splenocyt e proliferation. The in vitro antibody response against sheep red bloo d cells (anti-SRBC) was inhibited by ethanol extracts and quaternary a lkaloid fraction. The intraperitoneal (i.p.) application of ethanol ex tract and tertiary alkaloid fraction from aerial parts showed that the y possess in vivo effect on alternative pathway (AP) complement activi ty, anti-SRBC response and delayed type hypersensitivity (DTH).