A PROCEDURE TO STUDY UNSCHEDULED DNA-SYNTHESIS IN RAT SPERMATOGENIC CELLS IN-VIVO - STRAIN DIFFERENCES BETWEEN FISHER-344 AND SPRAGUE-DAWLEY RATS

Authors
SOTOMAYOR RE JACKSON R NOURBAKHSH A
Citation
Re. Sotomayor et al., A PROCEDURE TO STUDY UNSCHEDULED DNA-SYNTHESIS IN RAT SPERMATOGENIC CELLS IN-VIVO - STRAIN DIFFERENCES BETWEEN FISHER-344 AND SPRAGUE-DAWLEY RATS, Toxicology letters, 89(1), 1996, pp. 77-81
Citations number
18
Categorie Soggetti
Toxicology
Journal title
Toxicology letters → ACNP
ISSN journal
03784274
Volume
89
Issue
1
Year of publication
1996
Pages
77 - 81
Database
ISI
SICI code
0378-4274(1996)89:1<77:APTSUD>2.0.ZU;2-V
Abstract
A liquid scintillation counting (LSC) procedure was used to study unsc heduled DNA synthesis (UDS) in pachytene spermatocytes and spermatids from Fisher-344 (F344) and Sprague-Dawley (SPD) rats treated with meth yl methanesulfonate (MMS). MMS induced a large, dose-dependent, UDS re sponse in pachytene spermatocytes from both rat strains. On average, F 344 pachytene spermatocytes showed a larger UDS response than those of SPD rats. The lowest dose of MMS that elicited a significant UDS resp onse was 1 mg/kg in F344 rats but 5 mg/kg in SPD rats. Early spermatid stages from F344 rats also showed a larger UDS response than those fr om SPD rats. The time interval at which spermatid stages showed the ma ximum UDS response was between 20 and 24 days after MMS treatment. It is concluded that UDS can be measured quantitatively in rat spermatoge nic cells in vivo by using the LSC procedure.