HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF FLUCONAZOLE IN PLASMA

A high-performance liquid chromatographic method with ultraviolet abso rbance detection at 260 nm was developed for the analysis of fluconazo le in plasma. The method involves sample clean-up by liquid-liquid ext raction. The proposed technique is reproducible, selective, reliable a nd sensitive. Calibration standards were prepared in the range 1.25-20 mg/l. The limit of quantitation was 0.4 mg/l. The coefficients of var iation were 5% between measurements of a single extract injected in du plicate, and 7% between two extractions of spiked samples at the same concentrations. The separation between fluconazole and endogenous subs tances was satisfactory. This method was designed in order to minimise the risk of interference from substances that could be co-administere d to critically ill patients undergoing hemodiafiltration. With a run time below 5 min, the present method is rapid and easy to use for late r clinical studies, as well as for routine monitoring.