VALIDATED CAPILLARY ELECTROPHORETIC METHOD FOR THE QUANTITATIVE-ANALYSIS OF HISTAMINE ACID PHOSPHATE AND OR BENZALKONIUM CHLORIDE/

A novel capillary electrophoresis method has been developed and valida ted for the quantitative determination of histamine acid phosphate (HA P) and/or benzalkonium chloride (BKC). The solutes were separated usin g a pH 2.5 phosphate electrolyte with detection at 200 nm. Acceptable precision was obtained using internal standardisation. The method was also acceptable for determining levels of histidine which is an impuri ty in HAP. Profiling of BKC homologues was demonstrated for batch iden tity purposes. This method is used routinely and it is intended to reg ister this method in the British Pharmacopoeia to supplement current t est methods of TLC and HPLC.