THE INTRACELLULAR CYTOPLASMIC DOMAIN OF THE ALZHEIMERS-DISEASE AMYLOID PRECURSOR PROTEIN INTERACTS WITH PHOSPHOTYROSINE-BINDING DOMAIN PROTEINS IN THE YEAST 2-HYBRID SYSTEM

We have used the yeast two-hybrid system to screen for proteins that i nteract with the carboxy-terminal domain of APP, Six different clones mere isolated and sequence analyses revealed that they encoded domains of a previously described neuronal protein Fe65, a homologue of Fe65 and a homologue of protein X11, All of these proteins contain one or m ore phosphotyrosine binding (PTB) domains, PTB domain proteins bind to the sequence Asn-Pro-X-Tyr when the Tyr is phosphorylated and are bel ieved to function in signal transduction, APP contains such a motif, T hese results are consistent with a role for APP in signal transduction mechanisms.