RECOMBINANT FACTOR-IX SECRETED BY TRANSDUCED HUMAN KERATINOCYTES IS BIOLOGICALLY-ACTIVE

Authors
GERRARD AJ AUSTEN DEG BROWNLEE GG
Citation
Aj. Gerrard et al., RECOMBINANT FACTOR-IX SECRETED BY TRANSDUCED HUMAN KERATINOCYTES IS BIOLOGICALLY-ACTIVE, British Journal of Haematology, 95(3), 1996, pp. 561-563
Citations number
12
Categorie Soggetti
Hematology
Journal title
British Journal of Haematology → ACNP
ISSN journal
00071048
Volume
95
Issue
3
Year of publication
1996
Pages
561 - 563
Database
ISI
SICI code
0007-1048(1996)95:3<561:RFSBTH>2.0.ZU;2-R
Abstract
We have been investigating the use of human keratinocytes as potential target cells for gene therapy for haemophilia B, with the aim of curi ng haemophilia by means of factor IX secreting skin graft. Previous st udies indicated that keratinocytes might be suitable cells, although a potential problem was that the recombinant factor IX secreted by tran sduced keratinocytes was found to be only 40% biologically active, We now report, using an alternative assay to test for activity, that the secreted factor IX appears to be almost fully active.