K. Akasaka et al., HIGHLY SENSITIVE FLOW-INJECTION ANALYSIS OF LIPID HYDROPEROXIDES IN FOODSTUFFS, Bioscience, biotechnology, and biochemistry, 60(11), 1996, pp. 1772-1775
Lipid hydroperoxides in oils and foods were measured by a flow injecti
on analysis system with high sensitivity and selectivity. After sample
injection, lipid hydroperoxides were reacted with diphenyl-1-pyrenylp
hosphine (DPPP) in a stainless steel coil, then the fluorescence inten
sity of DPPP oxide, that was produced by the reaction, was monitored.
By this method, trilinolein hydroperoxide showed good linearity betwee
n 0.4 and 79 pmol and their detection limits were 0.2 pmol (signal-to-
noise ratio=3). The method made it possible to inject samples at 2-min
intervals. There was a good agreement of the amounts of lipid hydrope
roxides in oils and foods between by the batch method with DPPP and by
the proposed method (coefficient of correlation: r=0.999; n=21; perox
ide value=0.09-167 meq/g). With this method, the calibration graph of
trilinolein hydroperoxide was useful for all samples tested.