GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1

Authors
KOBAYASHI K KATO M MIURA Y KETTOKU M KOMEDA T IWAMATSU A
Citation
K. Kobayashi et al., GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1, Bioscience, biotechnology, and biochemistry, 60(11), 1996, pp. 1882-1885
Citations number
18
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
11
Year of publication
1996
Pages
1882 - 1885
Database
ISI
SICI code
0916-8451(1996)60:11<1882:GCAEON>2.0.ZU;2-W
Abstract
The genes encoding for trehalose-producing enzymes, a glycosyl-trehalo se-producing enzyme (glycosyltransferase) and a glycosyl-trehalose-hyd rolyzing enzyme (alpha-amylase), from Sulfolobus sol-fataricus KM1 wer e cloned and expressed in E. coli. The nucleotide sequence of the glyc osyltransferase gene and the alpha-amylase gene indicated proteins wit h lengths of 728 and 558 amino acids and molecular masses of 86-kDa an d 65-kDa, respectively. Regions highly conserved in the alpha-amylase family exist in the amino acid sequences of these enzymes.