PREPARATION OF SPECIFIC ANTISERUM TO 17-ALPHA-ESTRADIOL 17-N-ACETYLGLUCOSAMINIDE

A specific enzyme immunoassay (EIA) method has been developed for 17 a lpha-estradiol 17-N-acetylglucosaminide as a model compound instead of 15 alpha-hydroxyestrogen 15-N-acetylglucosaminides. Two new haptens, 3-(omega-carboxyalkyl) ether derivatives of 17 alpha-estradiol 17-N-ac etylglucosaminide, were synthesized and conjugated with bovine serum a lbumin (BSA). The EIA system was newly established using specific anti serum elicited against 3-(1-carboxypropyl) ether of 17-alpha-estradiol 17-N-acetylglucosaminide (17NAG CPE)-BSA conjugate and beta-galactosi dase-labeled 17NAG CPE as a labeled antigen. An appropriate dose-respo nse curve of EIA for 17 alpha-estradiol 17-N-acetylglucosaminide was o btained in the range of 20-1000 pg/tube. The specificity of EIA proved to be satisfactory in terms of cross-reactivities to related compound s including 15 alpha-N-acetylglucosaminides. The proposed method will be applicable to the preparation of antisera for use in EIA of 15 alph a-hydroxyesterogen 15-N-acetylglucosaminides.