EVOLUTION OF A SPECIFIC FLUOROGENIC DERIVATIZATION OF IVERMECTIN FOR BIOANALYTICAL APPLICATIONS - A REVIEW

The evolution of the fluorogenic derivatization of ivermectin is trace d through a series of continual modifications that have resulted in im provements in speed and sensitivity, Since the original development of this selective analytical technique, the reaction time has been short ened from 24 h at 100 degrees C to < 30 s at room temperature and, thr ough modifications of the derivatization reagent and catalyst, the sen sitivity has also been increased 50-fold to 20 pg of analyte with no s ignificant decrease in precision, ii procedure is reported, based on t he use of fluorescence derivatization, which eliminates the use of sol id-phase columns for sample preparation and fluorophore isolation, and is faster and less cumbersome than previous methods, The method was e valuated with cattle and canine plasma samples over the concentration range 1.0-40 ng ml(-1) of ivermectin, It has an accuracy of 1.9% (mean relative error) over this concentration range and a precision of 5.6% (RSD) at the 1 ng ml(-1) ivermectin concentration level in a 1 ml pla sma sample.