Dw. Fink et al., EVOLUTION OF A SPECIFIC FLUOROGENIC DERIVATIZATION OF IVERMECTIN FOR BIOANALYTICAL APPLICATIONS - A REVIEW, Analyst, 121(11), 1996, pp. 1533-1537
The evolution of the fluorogenic derivatization of ivermectin is trace
d through a series of continual modifications that have resulted in im
provements in speed and sensitivity, Since the original development of
this selective analytical technique, the reaction time has been short
ened from 24 h at 100 degrees C to < 30 s at room temperature and, thr
ough modifications of the derivatization reagent and catalyst, the sen
sitivity has also been increased 50-fold to 20 pg of analyte with no s
ignificant decrease in precision, ii procedure is reported, based on t
he use of fluorescence derivatization, which eliminates the use of sol
id-phase columns for sample preparation and fluorophore isolation, and
is faster and less cumbersome than previous methods, The method was e
valuated with cattle and canine plasma samples over the concentration
range 1.0-40 ng ml(-1) of ivermectin, It has an accuracy of 1.9% (mean
relative error) over this concentration range and a precision of 5.6%
(RSD) at the 1 ng ml(-1) ivermectin concentration level in a 1 ml pla
sma sample.