REGULATION OF M-TYPE POTASSIUM CHANNELS IN MAMMALIAN SYMPATHETIC NEURONS - ACTION OF INTRACELLULAR CALCIUM ON SINGLE-CHANNEL CURRENTS

Currents through single M-type potassium channels were recorded in mem brane patches excised from rat superior cervical sympathetic neurons. Application of Ca2+ to the internal face of inside-out patches produce two forms of M-channel inhibition: a slow, all-or-nothing suppression of activity; and a fast block associated with a concentration-depende nt shortening of open times compatible with open-channel block. Both f orms of block were enhanced by patch depolarization. Neither was repli cated or affected by Mg2+, and both could be recorded in the absence o f intracellular ATP, implying that they did not involve phosphorylatio n. Since the block was reversible in the absence of ATP and since alka line phosphatase did not reduce channel activity, block was unlikely t o have resulted from dephosphorylation. In cell-attached patch recordi ngs, M-channel activity increased during exposure of the cell to Ca2+- free solution and was rapidly reduced on applying 2 mM Ca2+ to the ext ra-patch solution. This suggests that M-channel activity in these neur ons may be tonically regulated by variations in resting intracellular [Ca2+]. Copyright (C) 1996 Elsevier Science Ltd