LIGHT-DIRECTED SYNTHESIS OF HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS USINGSEMICONDUCTOR PHOTORESISTS

Citation
G. Mcgall et al., LIGHT-DIRECTED SYNTHESIS OF HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS USINGSEMICONDUCTOR PHOTORESISTS, Proceedings of the National Academy of Sciences of the United Statesof America, 93(24), 1996, pp. 13555-13560
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
93
Issue
24
Year of publication
1996
Pages
13555 - 13560
Database
ISI
SICI code
0027-8424(1996)93:24<13555:LSOHOA>2.0.ZU;2-9
Abstract
High-density arrays of oligonucleotide probes are proving to be powerf ul new tools for large-scale DNA and RNA sequence analysis. A method f or constructing these arrays, using light-directed DNA synthesis with photoactivatable monomers, can currently achieve densities on the orde r of 10(6) sequences/cm(2). One of the challenges facing this technolo gy is to further increase the volume, complexity, and density of seque nce information encoded in these arrays, Here we demonstrate a new app roach for synthesizing DNA probe arrays that combines standard solid-p hase oligonucleotide synthesis with polymeric photoresist films servin g as the photoimageable component. This opens the way to exploiting hi gh-resolution imaging materials and processes from the microelectronic s industry for the fabrication of DNA probe arrays with substantially higher densities than are currently available.