G. Mcgall et al., LIGHT-DIRECTED SYNTHESIS OF HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS USINGSEMICONDUCTOR PHOTORESISTS, Proceedings of the National Academy of Sciences of the United Statesof America, 93(24), 1996, pp. 13555-13560
High-density arrays of oligonucleotide probes are proving to be powerf
ul new tools for large-scale DNA and RNA sequence analysis. A method f
or constructing these arrays, using light-directed DNA synthesis with
photoactivatable monomers, can currently achieve densities on the orde
r of 10(6) sequences/cm(2). One of the challenges facing this technolo
gy is to further increase the volume, complexity, and density of seque
nce information encoded in these arrays, Here we demonstrate a new app
roach for synthesizing DNA probe arrays that combines standard solid-p
hase oligonucleotide synthesis with polymeric photoresist films servin
g as the photoimageable component. This opens the way to exploiting hi
gh-resolution imaging materials and processes from the microelectronic
s industry for the fabrication of DNA probe arrays with substantially
higher densities than are currently available.