ADDITION OF PHOSPHATIDYLCHOLINE-PHOSPHOLIPASE-C INDUCES CELLULAR REDISTRIBUTION AND PHOSPHORYLATION OF PROTEIN-KINASE-C-ZETA IN C-6 GLIAL-CELLS

Citation
I. Galveroperh et al., ADDITION OF PHOSPHATIDYLCHOLINE-PHOSPHOLIPASE-C INDUCES CELLULAR REDISTRIBUTION AND PHOSPHORYLATION OF PROTEIN-KINASE-C-ZETA IN C-6 GLIAL-CELLS, Neuroscience letters, 219(1), 1996, pp. 68-70
Citations number
19
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
03043940
Volume
219
Issue
1
Year of publication
1996
Pages
68 - 70
Database
ISI
SICI code
0304-3940(1996)219:1<68:AOPICR>2.0.ZU;2-8
Abstract
Phosphatidylcholine breakdown has been shown to play a critical role i n signal transduction involving generation of a number of second messe ngers [Exton, J.H., Biochim. Biophys. Acta, 1212 (1994) 26-42]. In the present report we demonstrate by immunofluorescence that short-treatm ent of C6 glial cells with phosphatidylcholine-hydrolyzing phospholipa se C (PC-PLC), changes the intracellular localization of protein kinas e C (PKC) zeta from the cytoplasm to a perinuclear region, Western blo t analysis also showed a redistribution of PKC zeta after incubation o f cells with PC-PLC. To test whether these changes were accompanied by an activation of the enzyme, we measured the extent of phosphorylatio n of PKC zeta by immunoprecipitation from P-32-labelled cells, Short-t reatment with PC-PLC resulted in enhanced phosphorylation of the highe r Mr PKC zeta in C6 glial cells.