I. Galveroperh et al., ADDITION OF PHOSPHATIDYLCHOLINE-PHOSPHOLIPASE-C INDUCES CELLULAR REDISTRIBUTION AND PHOSPHORYLATION OF PROTEIN-KINASE-C-ZETA IN C-6 GLIAL-CELLS, Neuroscience letters, 219(1), 1996, pp. 68-70
Phosphatidylcholine breakdown has been shown to play a critical role i
n signal transduction involving generation of a number of second messe
ngers [Exton, J.H., Biochim. Biophys. Acta, 1212 (1994) 26-42]. In the
present report we demonstrate by immunofluorescence that short-treatm
ent of C6 glial cells with phosphatidylcholine-hydrolyzing phospholipa
se C (PC-PLC), changes the intracellular localization of protein kinas
e C (PKC) zeta from the cytoplasm to a perinuclear region, Western blo
t analysis also showed a redistribution of PKC zeta after incubation o
f cells with PC-PLC. To test whether these changes were accompanied by
an activation of the enzyme, we measured the extent of phosphorylatio
n of PKC zeta by immunoprecipitation from P-32-labelled cells, Short-t
reatment with PC-PLC resulted in enhanced phosphorylation of the highe
r Mr PKC zeta in C6 glial cells.