ELECTRICAL COMMUNICATION BETWEEN ELECTRODES AND ENZYMES MEDIATED BY REDOX HYDROGELS

Different redox polymers based on poly(allylamine) with covalently att ached ferrocene and pyridine groups that coordinate iron and ruthenium complexes were prepared, and hydrogels were obtained by cross-linking them with epichlorohydrin. Charge propagation from the underlying ele ctrode, through the redox polymer and electrical communication with th e enzyme FADH(2) of glucose oxidase, was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The effects of electrolyt e composition, concentration of enzyme and substrate, and electrode po tential are reported, The role of different redox mediators covalently attached to the polymer back-bone is discussed in terms of driving fo rce and electrostatic barriers.