EFFECT OF STORAGE TIME ON THE IMMUNOREACTIVITY OF IGG PHYSICALLY ADSORBED OR CHEMICALLY BOUND TO LATEX BEADS

The main purpose of the present work is to check whether the immunorea ctivity of IgG molecules, chemically bound or adsorbed on latex, shows alteration after 6 months of storage, For this purpose, agglutination tests of sensitized carboxylated beads, measuring the immunoreactivit y by turbidimetric monitoring after 5 min of immunoaggregation reactio n with human C-reactive protein in a stopped-flow spectrophotometer, w ere repeated, The results show that while the covalently bound IgG mai ntains and in some cases improves on its previous immunological respon se, the passively adsorbed IgG shows loss of activity due to a decreas ed amount of adsorbed protein. (C) 1996 Academic Press, Inc.