CALIBRATION OF GEL-PERMEATION COLUMNS IN THE HIGH-MOLECULAR-MASS RANGE - FIXED HUMAN THROMBOCYTES FOR THE ESTIMATION OF INTERSTITIAL VOLUMEAND THE HEMOCYANIN OF THE VINEYARD SNAIL HELIX-POMATIA AS A MOLECULAR-MASS CALIBRATION SUBSTANCE
Wkr. Barnikol et H. Potzschke, CALIBRATION OF GEL-PERMEATION COLUMNS IN THE HIGH-MOLECULAR-MASS RANGE - FIXED HUMAN THROMBOCYTES FOR THE ESTIMATION OF INTERSTITIAL VOLUMEAND THE HEMOCYANIN OF THE VINEYARD SNAIL HELIX-POMATIA AS A MOLECULAR-MASS CALIBRATION SUBSTANCE, Journal of chromatography, 685(2), 1994, pp. 221-227
Human thrombocytes, fixed with formaldehyde, are shown to be a univers
al indicator of the interstitial volume in gel-permeation chromatograp
hy (size-exclusion chromatography) for gels of all molecular mass sepa
ration ranges. The fixed thrombocytes are simple to prepare and to han
dle. Furthermore, native or intramolecularly cross-linked haemocyanin
of the Roman snail Helix pomatia is shown to be well suited as a molec
ular mass calibration substance. We propose 8.8 10(6) as its molecular
mass for calibration. This haemocyanin can therefore be added to the
list of globular protein molecular mass calibration substances in the
range of very high molecular masses.