FRACTIONATION OF NON-ORTHO-SUBSTITUTED TOXIC POLYCHLORINATED-BIPHENYLS ON 2 NITRO-CONTAINING LIQUID-CHROMATOGRAPHIC STATIONARY PHASES

Two liquid chromatographic stationary phases, dinitroanilinopropylsili ca (DNAP) and tetranitrofluoreniminopropylsilica (TENF), are demonstra ted to retain selectively the toxic non-ortho-chlorobiphenyl congeners CB-77, CB-126 and CB-169. On the DNAP column, these three congeners e lute as a single peak. With hexane as mobile phase all eighteen tested chlorobiphenyl congeners are eluted within 13 min. Carryover effects of CBs in HPLC systems are discussed. To demonstrate the isolation of the three congeners, a sample of Aroclor 1254 was fractionated and ana lysed by GC with electron-capture detection.