MEMBRANE AFFINITY-CHROMATOGRAPHY OF ALKALINE-PHOSPHATASE

A method for the preparation of a microporous cellulose membrane is de scribed. The triazine dyes Cibacron Blue F3GA and Active Red K2BP were immobilized as affinity ligands. Up to 90 mg of Active Red K2BP can b e coupled to 1 g of membrane matrix. A membrane cartridge containing r ed affinity membranes was also prepared. The flux of the cartridge con taining 80 sheets of membrane can reach 5.0 ml/min with a pressure dro p of 0.1 MPa. On this cartridge, the chromatography of alkaline phosph atase was performed with a 60% recovery of activity and a 40-fold puri fication.