A QUALITATIVE IN-VITRO EVALUATION OF THE DEGRADABLE MATERIALS POLY(CAPROLACTONE) - POLY(HYDROXYBUTYRATE) AND A POLY(HYDROXYBUTYRATE)-(HYDROXYVALERATE) COPOLYMER
N. Gurav et S. Downes, A QUALITATIVE IN-VITRO EVALUATION OF THE DEGRADABLE MATERIALS POLY(CAPROLACTONE) - POLY(HYDROXYBUTYRATE) AND A POLY(HYDROXYBUTYRATE)-(HYDROXYVALERATE) COPOLYMER, Journal of materials science. Materials in medicine, 5(11), 1994, pp. 784-787
A qualitative in vitro evaluation of poly(caprolactone) (PCL), poly (h
ydroxybutyrate) (PHB) and a poly (hydroxybutyrate)-(hydroxyvalerate) (
PHB-PHV) copolymer was carried out using primary human osteoblasts (HO
B) and a human osteosarcoma (HOS) cell line. The cells were grown on f
ilms of these polymers and cultured for 2 and 4 days with cells grown
on Thermanox as a control. The cells on each of the polymers exhibited
different cellular morphologies with different rates of cell prolifer
ation. Results from a preliminary degradation study demonstrated that
biodegradable materials can be partially degraded using enzymes such a
s papain and trypsin. Of the solutions tested, papain caused the great
est degradation, with phosphate-buffered saline (PBS) a physiological
buffer having very little effect over a six week period. The bone cell
s were grown on partially degraded polymers and no differences in the
performance of HOS and HOB cells on the materials were observed.