STRUCTURAL DETERMINANTS OF PUTRESCINE UPTAKE INHIBITION PRODUCED BY CATIONIC DIAMIDINES IN THE MODEL OF TRYPANOSOMATID CRITHIDIA-FASCICULATA

The effect of a heterologous series of cationic diamidines has been te sted on cell growth and polyamine uptake on the model of insect Trypan osomatid Crithidia fasciculata. The maximum inhibitory effect on both parameters was found for pentamidine and dibromopropamidine, which exh ibit a longer distance between amino and imino substituents. A minimum inhibitory effect was found with amicarbalide. A good relationship wa s obtained when the distance between amino moieties was plotted versus the inhibitory effect on putrescine uptake, suggesting a role of this structural property on polyamine transport in Crithidia fasciculata. In addition, a similar correlation was obtained for another Trypanosom atid parasite, Leishmania infantum.