Yd. Hang et Ee. Woodams, APPL POMACE - A POTENTIAL SUBSTRATE FOR PRODUCTION OF BETA-GLUCOSIDASE BY ASPERGILLUS-FOETIDUS, Lebensmittel-Wissenschaft + Technologie, 27(6), 1994, pp. 587-589
Of the three Aspergillus species examined, Aspergillus foetidus NRRL 3
37 was found to produce more beta-glucosidase activity on apple pomace
than Aspergillus niger NRRL 599 and Aspergillus fumigatus P1061. The
enzyme was purified 12-fold by ultrafiltration and gel filtration with
a yield of 490 g/kg. The partially purified enzyme exhibited its opti
mal activity at 65-degrees-C and was most active at pH 4.6. The K(m) v
alue for p-nitrophenol beta D-glucopyranoside (PNPG) was 0.87 mmol/L.
The enzyme had a molecular weight of 275 kDa as estimated by gel filtr
ation with Sephadex G-200.