Splicing is a new powerful tool, stemming originally from molecular ge
netics but investigated extensively also in language theory. In this p
aper we investigate variants of splicing inspired partly by regulating
mechanisms customarily studied in language theory, partly by imposing
restrictions on the pairs to be spliced or on the result of splicing.
The Chomsky hierarchy constitutes a very suitable test bed for the re
sulting families, because it is classical and well understood. In cont
rast to the usual, nonrestricted splicing, we find several cases when
the families of regular or of context-free languages are not closed un
der the new types of splicing. On the other hand, our results give new
characterizations for families in the Chomsky hierarchy and for closu
re properties in general.