TEMPERATURE AS A VARIABLE IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATIONS OF PEPTIDE AND PROTEIN SAMPLES .1. OPTIMIZING THE SEPARATION OF A GROWTH-HORMONE TRYPTIC DIGEST

Peptide and protein samples are often complex mixtures that contain a number of individual compounds. The initial HPLC separation of such sa mples typically results in the poor resolution of one or more band pai rs. Various means have been suggested for varying separation selectivi ty so as to minimize this problem. In this study of a tryptic digest o f recombinant human growth hormone, the simultaneous variation of temp erature and gradient ;steepness was found to be a convenient and effec tive means of varying selectivity and optimizing the separation. The u se of computer simulation greatly facilitated this investigation.