RELATIONSHIP BETWEEN THE INDUCTION OF PROTEINS IN THE HSP70 FAMILY AND THERMOSENSITIVITY IN 2 SPECIES OF ORYZIAS (PISCES)

A cultured fish cell line, CE, derived from Oryzias celebensis, which lives in a tropical zone, was more heat-resistant than the OL32, which were derived from the Japanese medaka, Oryzias latipes which lives in a temperate zone. Protein synthesis in OL32 cells was also more heat- sensitive than that in CE cells. The relative levels in proteins of th e HSP70 family and the ability of cells to tolerate severe heat treatm ent after a conditioning heat treatment were examined. Twenty-four hou rs after conditioning heat treatment, both cell lines retained thermot olerance even though three proteins in the HSP70 family had returned t o their control levels.