G. Degani et al., IN-VITRO BIOSYNTHESIS OF STEROIDS IN OVARY OF ASYNCHRONIC TRICHOGASTER-TRICHOPTERUS (PALLUS, 1770), Comparative biochemistry and physiology. B. Comparative biochemistry, 109(4), 1994, pp. 715-723
The relationship between ovarian steroidogenesis and the development o
f oogenesis was studied in the ovary of Trichogaster trichopterus by h
istological examination and thin layer chromotography. The percent yie
ld of 17 beta-estradiol (E(2)) was high at a number of different stage
s of vitellogenesis, while those of 17 alpha,20 beta-dikydroxy-4-pregn
en-3-one (17,20-P) and testosterone (T) were high at the beginning of
maturation, and increased steadily during this process. The per cent y
ields of T and 17 alpha-hydroxyprogesterone (17-P) were low at all sta
ges of vitellogenesis (VTG). For androstenedione (AS), 5-androstane-3
beta-ol-17-one, 5 beta-pregnane-3 alpha,17 alpha,20 alpha-triol and 11
-ketotestosterone, the per cent yields appeared to increase mainly dur
ing maturation and ovulation. It is suggested that the main pathways o
f steroidogenesis during oogenesis are: at VTG, [P]-->[17-P]-->[AS]-->
[E(2)]; and at maturation [17-P]-->[17,20-P], the latter controlled by
male stimulation of GtH.