IN-VITRO BIOSYNTHESIS OF STEROIDS IN OVARY OF ASYNCHRONIC TRICHOGASTER-TRICHOPTERUS (PALLUS, 1770)

Citation
G. Degani et al., IN-VITRO BIOSYNTHESIS OF STEROIDS IN OVARY OF ASYNCHRONIC TRICHOGASTER-TRICHOPTERUS (PALLUS, 1770), Comparative biochemistry and physiology. B. Comparative biochemistry, 109(4), 1994, pp. 715-723
Citations number
38
Categorie Soggetti
Biology
ISSN journal
03050491
Volume
109
Issue
4
Year of publication
1994
Pages
715 - 723
Database
ISI
SICI code
0305-0491(1994)109:4<715:IBOSIO>2.0.ZU;2-A
Abstract
The relationship between ovarian steroidogenesis and the development o f oogenesis was studied in the ovary of Trichogaster trichopterus by h istological examination and thin layer chromotography. The percent yie ld of 17 beta-estradiol (E(2)) was high at a number of different stage s of vitellogenesis, while those of 17 alpha,20 beta-dikydroxy-4-pregn en-3-one (17,20-P) and testosterone (T) were high at the beginning of maturation, and increased steadily during this process. The per cent y ields of T and 17 alpha-hydroxyprogesterone (17-P) were low at all sta ges of vitellogenesis (VTG). For androstenedione (AS), 5-androstane-3 beta-ol-17-one, 5 beta-pregnane-3 alpha,17 alpha,20 alpha-triol and 11 -ketotestosterone, the per cent yields appeared to increase mainly dur ing maturation and ovulation. It is suggested that the main pathways o f steroidogenesis during oogenesis are: at VTG, [P]-->[17-P]-->[AS]--> [E(2)]; and at maturation [17-P]-->[17,20-P], the latter controlled by male stimulation of GtH.