SEPARATION OF CHLOROPHYLL C(1) AND C(2) BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Successful separation of chlorophyll c(1) (Chl-c(1)), chlorophyll c(2) (Chl-c(2)) and their demetallated forms (pheoporphyrins) was performe d by the electrokinetic chromatographic mode of capillary zone electro phoresis in a running solution containing micelles of sodium dodecyl s ulfate and dimethylformamide. Chl-c(1) migrated with higher velocity t han Chl-c(2), and their migration times were shorter than those of the corresponding demetallated forms. Complete resolution was obtained fo r these compounds within 20 min under the conditions of migration dist ance (i.e., effective capillary length) 50 cm and electric field 429 V /cm. Application to the separation of chlorophylls in a typical brown seaweed was demonstrated.